| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222949 |
| Chromosome: | chromosome 3 |
| Location: | 8607511 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203121 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTGGCCCACCCGCACTGCAGATTTGGGA |
| Internal bar code: | CGCCTGTATAGGAGGTTTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1018 |
| LEAP-Seq percent confirming: | 99.6547 |
| LEAP-Seq n confirming: | 12699 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACTGAAACAGTACGGGG |
| Suggested primer 2: | ATAGCAGAGTTTGGAGGCGA |