Insertion junction: LMJ.RY0402.222956_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.222956
Chromosome:chromosome 3
Location:4332475
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g174950 TGL7 (1 of 3) PTHR21493//PTHR21493:SF87 - CGI-141-RELATED/LIPASE CONTAINING PROTEIN // SN1-SPECIFIC DIACYLGLYCEROL LIPASE BETA; Putative triacylglycerol lipase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCACATCCCCACCACCCGAAAATCTTCG
Internal bar code:TTTAACTTGCACCCACACGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:312
LEAP-Seq percent confirming:87.0842
LEAP-Seq n confirming:445
LEAP-Seq n nonconfirming:66
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGTGGTTTGACAGGGATT
Suggested primer 2:GAAGCAATTTACGGCTTTGC