| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.222957 |
| Chromosome: | scaffold 18 |
| Location: | 91195 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g748597 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCTCGCCAGCCTCGCCTTTCATTAGGGC |
| Internal bar code: | CTCACGACGAAGCTCGAAATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 980 |
| LEAP-Seq percent confirming: | 99.7907 |
| LEAP-Seq n confirming: | 6199 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTGGTTTGGGATACTTT |
| Suggested primer 2: | ACCAAAGTGCAAAAGATGGG |