Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.222966 |
Chromosome: | chromosome_9 |
Location: | 3668673 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g390615 | FAP12 | Triacylglycerol lipase and Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGGGTCCTCAGTGGCCGCCAGGTGGTCAGG |
Internal bar code: | CCTGGGACACATATCCATTTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 531 |
LEAP-Seq percent confirming: | 96.6713 |
LEAP-Seq n confirming: | 697 |
LEAP-Seq n nonconfirming: | 24 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGAATGGATGGAGGAGGAG |
Suggested primer 2: | AAGTTGCTCTAAGGCCGACA |