| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.223001 |
| Chromosome: | chromosome 3 |
| Location: | 2934573 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163150 | (1 of 2) K09955 - hypothetical protein (K09955) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCAGCGGCGGCCGCGGCAGCGGCAAGGG |
| Internal bar code: | ACGACCGGGTTTGTCTGGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 6.25 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTAAGGTGAACAGCCGC |
| Suggested primer 2: | AGTCAATGCGAAGCCCTAGA |