Insertion junction: LMJ.RY0402.223018_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.223018
Chromosome:chromosome 12
Location:7950449
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g552300 CTL3 (1 of 1) IPR001304//IPR016187//IPR024616 - C-type lectin // C-type lectin fold // Pherophorin; Putative pherophorin with C-type lectin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAATGTCATAGCAGCCCGGCGCGCCGGTC
Internal bar code:ACGGCGCTTGACTGTCGTTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1046
LEAP-Seq percent confirming:99.2664
LEAP-Seq n confirming:7307
LEAP-Seq n nonconfirming:54
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGCCCACTCTATTACCCG
Suggested primer 2:TACCCCGGTGAAACCAATAA