Insertion junction: LMJ.RY0402.223018_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.223018
Chromosome:chromosome 12
Location:7950499
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g552300 CTL3 (1 of 1) IPR001304//IPR016187//IPR024616 - C-type lectin // C-type lectin fold // Pherophorin; Putative pherophorin with C-type lectin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGGGAGCTGGCGACAGGTTGCCTGCGCC
Internal bar code:GCACCGTCCTGACAAGGTGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:588
LEAP-Seq percent confirming:99.3627
LEAP-Seq n confirming:1871
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGCCCACTCTATTACCCG
Suggested primer 2:TACCCCGGTGAAACCAATAA