Insertion junction: LMJ.RY0402.223021_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.223021
Chromosome:chromosome_8
Location:4151435
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre08.g379900 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GCGCCGTACGCTCGTCACCGTACGACAGAG
Internal bar code:TTTCGACTTCCTGAAAATACT

Confirmation - LEAP-Seq

LEAP-Seq distance:1051
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:77
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTAACGACAGCTTGCGAC
Suggested primer 2:TTTTCCTGTGCTTTCCTGCT