| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.223025 |
| Chromosome: | chromosome 6 |
| Location: | 1418321 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259050 | (1 of 1) PTHR24365:SF279 - PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGCGAAGTGTGAGCATCAGAGAATTGT |
| Internal bar code: | TTTTTGGGGTGGACGCTTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 951 |
| LEAP-Seq percent confirming: | 99.0269 |
| LEAP-Seq n confirming: | 14451 |
| LEAP-Seq n nonconfirming: | 142 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACATGCAGTGTTGTGGGC |
| Suggested primer 2: | GAACCCGTGACTCCATGACT |