Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.223054 |
Chromosome: | chromosome_13 |
Location: | 55979 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g562250 | FAP92 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTTGGTCAGTGAGCTTACACGTGGCTTAAC |
Internal bar code: | GTGCCCAGTGAGCAATGCAGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 583 |
LEAP-Seq percent confirming: | 99.4466 |
LEAP-Seq n confirming: | 1258 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGGTGCTCCACACAAACC |
Suggested primer 2: | AACAAGCTCTTTTGAGGGCA |