| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.223058 |
| Chromosome: | chromosome 9 |
| Location: | 7081245 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411600 | (1 of 3) IPR000104//IPR001005//IPR009057//IPR017877//IPR017930 - Antifreeze protein, type I // SANT/Myb domain // Homeodomain-like // Myb-like domain // Myb domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATGGATTTGAACCTGGGGGGCCGAGGA |
| Internal bar code: | AGGCCTCGTGTCTGGCCTTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 108 |
| LEAP-Seq percent confirming: | 62.1212 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACGTTCTCTCCACGTTCT |
| Suggested primer 2: | GACTCGGAATGCTGGTGGT |