| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.223075 |
| Chromosome: | chromosome 13 |
| Location: | 3263563 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586000 | (1 of 1) K18660 - malonyl-CoA/methylmalonyl-CoA synthetase (ACSF3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCCCTGCGTTGTACAGCTTGAGCAGCT |
| Internal bar code: | GCGGGCAGACCGGTTCTATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 343 |
| LEAP-Seq percent confirming: | 99.5055 |
| LEAP-Seq n confirming: | 6842 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGGGACACTGCATGACT |
| Suggested primer 2: | GGTTTCAACCACATGCACAG |