| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.223088 |
| Chromosome: | chromosome 10 |
| Location: | 122451 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418600 | RLS3 | RegA/Rls-like protein; (1 of 10) IPR010919 - SAND domain-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCTGGCTGCCGACTGCGGCGCCCGCCG |
| Internal bar code: | CCGGTCCTCTCTTTTTAGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1057 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCAAGCAAAACAGATGA |
| Suggested primer 2: | ATGGTGGTGGTGGTACTGGT |