| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.223101 |
| Chromosome: | chromosome 6 |
| Location: | 1352172 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258700 | PYC1 | (1 of 1) 6.4.1.1 - Pyruvate carboxylase / Pyruvic carboxylase; Pyruvate carboxylase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATTGAATCAAGGCAGATCTGCGTGTGTT |
| Internal bar code: | TGTTTGGGGGAAACCTAGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 876 |
| LEAP-Seq percent confirming: | 97.2912 |
| LEAP-Seq n confirming: | 1724 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATTGATTTCTCCGCATCT |
| Suggested primer 2: | GATTGCAAGGATGGAAGGAA |