Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.223102 |
Chromosome: | chromosome_6 |
Location: | 7193826 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g297850 | DHC12 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCATGGTCATCCAGACGGCGCTGGCGGCGG |
Internal bar code: | TAGCGGGTCCTTTTTCAGCTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 354 |
LEAP-Seq percent confirming: | 99.2851 |
LEAP-Seq n confirming: | 6250 |
LEAP-Seq n nonconfirming: | 45 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCCACACGTCTCCTTTTGG |
Suggested primer 2: | TAGTGGCGTGTTGTGGACAT |