Insertion junction: LMJ.RY0402.223109_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.223109
Chromosome:chromosome 6
Location:6131616
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g290050 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTACTGGTACTAAACTTCAGCAGTACTGGT
Internal bar code:GCGTAAATTTTGGCAGCAGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:365
LEAP-Seq percent confirming:99.5992
LEAP-Seq n confirming:497
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GACTTGTGTGTATGCGGGTG
Suggested primer 2:CGCTACATGTCCTTCTGCCT