Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.223119 |
Chromosome: | chromosome_6 |
Location: | 5590580 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g285600 | RWP5,MID | RWP-RK transcription factor/minus dominance protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCAGGCTTGTCAGCTGCAGCCAAATGCAA |
Internal bar code: | CGTAACTATAGGGACGGGGGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 430 |
LEAP-Seq percent confirming: | 96.5633 |
LEAP-Seq n confirming: | 4299 |
LEAP-Seq n nonconfirming: | 153 |
LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCAAGTACTCGCTCGGGAC |
Suggested primer 2: | GCATGGATGACTTCCTGGAT |