| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.223135 |
| Chromosome: | chromosome 1 |
| Location: | 3480466 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022100 | (1 of 11) PF00632 - HECT-domain (ubiquitin-transferase) (HECT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGAGCGGGAGGCGTGAGGGGCGGTGGCG |
| Internal bar code: | ACAGCTTGGCAGTTCCTCGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 211 |
| LEAP-Seq percent confirming: | 40.609 |
| LEAP-Seq n confirming: | 1107 |
| LEAP-Seq n nonconfirming: | 1619 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCCACACACCTGCATGAT |
| Suggested primer 2: | CTCAAACACACCTCAAGCCA |