| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.223136 |
| Chromosome: | chromosome 12 |
| Location: | 7412425 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556750 | TIC32,TIC32-1,SDR22 | Short-chain dehydrogenase/reductase; (1 of 2) 1.1.1.330 - Very-long-chain 3-oxoacyl-CoA reductase / Very-long-chain beta-ketoacyl-CoA reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAGGCGCTGTTGGCGCGGGCGCTCAAGG |
| Internal bar code: | CCCTGCTGGGCTATAGTTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 844 |
| LEAP-Seq percent confirming: | 88.1013 |
| LEAP-Seq n confirming: | 2436 |
| LEAP-Seq n nonconfirming: | 329 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACTGTACTGTGAAGCCA |
| Suggested primer 2: | GATCTGAACTGGGAGAAGCG |