| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.223137 |
| Chromosome: | chromosome 1 |
| Location: | 597518 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003475 | (1 of 2) 3.4.24.35 - Gelatinase B / Type V collagenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACACGCACACACAGTCGTCCCCTCTGCCT |
| Internal bar code: | ATATATATAAGCCTATAAGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 507 |
| LEAP-Seq percent confirming: | 99.7184 |
| LEAP-Seq n confirming: | 8499 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGATCACTGGATGTGTCG |
| Suggested primer 2: | GTGTACGGACACGGTGAGTG |