| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.223153 |
| Chromosome: | chromosome 7 |
| Location: | 4810269 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g345850 | MRPL35,bL35m | Mitochondrial ribosomal protein L35 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGAAGGCACGCCTCACAACCCCGAGCTA |
| Internal bar code: | AGGCGATGTGCAGTTCGGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 99.6711 |
| LEAP-Seq n confirming: | 1212 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTTTTCAGCCACGAAGC |
| Suggested primer 2: | ATACCGCCTTCATCCAACAG |