| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.223156 |
| Chromosome: | chromosome 3 |
| Location: | 7375812 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206250 | (1 of 39) IPR009003 - Peptidase S1, PA clan | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTGCCCGCCTTGATGCCCGCGTCCATG |
| Internal bar code: | TGGAAACTCGGCACGACAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 99.5669 |
| LEAP-Seq n confirming: | 1839 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTGACAGATTCCGTTGA |
| Suggested primer 2: | CTATCCCGTCTACTCGCTGC |