| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.223156 |
| Chromosome: | chromosome 5 |
| Location: | 2157826 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234645 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCCAGCCAGCCATCTGGCTAACCAGT |
| Internal bar code: | AGAGGCGATGAGGTAAGAAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 121 |
| LEAP-Seq percent confirming: | 81.9672 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGCACAAATACGCCCTT |
| Suggested primer 2: | GCTGGAGACTCTGGATGAGG |