| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.223203 |
| Chromosome: | chromosome 3 |
| Location: | 310785 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144607 | (1 of 79) IPR011992 - EF-hand domain pair | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGATGGTTGGAGTGAGTGAGTGACACGC |
| Internal bar code: | GAGGCAGCGGTGCCGTGAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 99.6763 |
| LEAP-Seq n confirming: | 6467 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTGTTGCGGAAGTGATA |
| Suggested primer 2: | AGGACGGAGGAGAAGAGGAG |