| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.223211 |
| Chromosome: | chromosome 13 |
| Location: | 4856100 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g605550 | (1 of 6) 2.7.7.84 - 2'-5' oligoadenylate synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCGAGCACCGCAGGAGGCAACGGTGGG |
| Internal bar code: | AGGGATACTTTGAACCAGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 577 |
| LEAP-Seq percent confirming: | 99.3631 |
| LEAP-Seq n confirming: | 156 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCTGTCGGTCTTCGTGAT |
| Suggested primer 2: | GATGTGCAGCGGTAAACAGA |