| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.223215 |
| Chromosome: | chromosome 3 |
| Location: | 4408581 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175600 | (1 of 1) PTHR26392//PTHR26392:SF78 - MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 7-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCGCAGCCAGCGGCGGCGCGCCAGCCGG |
| Internal bar code: | CTGATCGTTAGCGCTGATCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 791 |
| LEAP-Seq percent confirming: | 88.8186 |
| LEAP-Seq n confirming: | 421 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGGGTAGCTCAGCCTTAT |
| Suggested primer 2: | GATGGGAGACGTTCATCGTT |