| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.223219 |
| Chromosome: | chromosome 3 |
| Location: | 8860122 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207041 | (1 of 26) IPR029058//IPR029059 - Alpha/Beta hydrolase fold // Alpha/beta hydrolase fold-5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCAATGGGCTGGGGGGACATCAGCAAAG |
| Internal bar code: | CTCACGAGCGGTGGTAGTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 795 |
| LEAP-Seq percent confirming: | 99.1264 |
| LEAP-Seq n confirming: | 2156 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCAGGACTTGGAGATGT |
| Suggested primer 2: | AGGCCTTCCTCCTCCATTTA |