| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.223232 |
| Chromosome: | chromosome 17 |
| Location: | 3301178 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723200 | FAP372 | Flagellar Associated EF Hand Protein 372 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTCCTTCAGGCGGAGCTGGAGACGACGG |
| Internal bar code: | CGAGTACGAGACGAGGGCGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 520 |
| LEAP-Seq percent confirming: | 97.6337 |
| LEAP-Seq n confirming: | 1898 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAGGAGCTGAATTGGAG |
| Suggested primer 2: | TTTACCAACAGCGCACAAAG |