| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.223247 |
| Chromosome: | chromosome 1 |
| Location: | 3982623 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025900 | (1 of 7) PF00415//PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1) // Regulator of chromosome condensation (RCC1) repeat (RCC1_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACTGGGGTGGGGGTGGGGGTGGGGGCGC |
| Internal bar code: | CTGACCAATGGAAAGGCACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 494 |
| LEAP-Seq percent confirming: | 91.7647 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGTCTTTTATGGACGGC |
| Suggested primer 2: | TACCAAAGGACTGGACCTCG |