Insertion junction: LMJ.RY0402.223247_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.223247
Chromosome:chromosome 1
Location:3982643
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g025900 (1 of 7) PF00415//PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1) // Regulator of chromosome condensation (RCC1) repeat (RCC1_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCACCCCAGGGCCTCCGCTCACTCACC
Internal bar code:CTTCCACATGTTTGTACATCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:141
LEAP-Seq percent confirming:39.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:61
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCGTCTTTTATGGACGGC
Suggested primer 2:TACCAAAGGACTGGACCTCG