| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.223269 |
| Chromosome: | chromosome 17 |
| Location: | 1465276 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g706850 | (1 of 1) PF08551 - Eukaryotic integral membrane protein (DUF1751) (DUF1751) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCTGGTATCCTCTCAACCCGCGCCTCT |
| Internal bar code: | GGATAAGACCGCGTGGTCTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 158 |
| LEAP-Seq percent confirming: | 94.6644 |
| LEAP-Seq n confirming: | 550 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCGCGGGTTTAACTTCA |
| Suggested primer 2: | ATTATGCAATCCAAGCCTGC |