| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.223270 |
| Chromosome: | chromosome 13 |
| Location: | 149828 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g563150 | PAB1,CGLD8 | Assembly factor for CF1 or F1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTACAGGCGGGACCAGCTGAGGCCAGGA |
| Internal bar code: | GCCTAGACCCTTGTTACGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 703 |
| LEAP-Seq percent confirming: | 98.5672 |
| LEAP-Seq n confirming: | 3027 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCAGCTAGCAGGATGTAG |
| Suggested primer 2: | AGGCATAGGCTGCGTTAGAA |