| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.223300 |
| Chromosome: | chromosome 6 |
| Location: | 7182289 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g297800 | (1 of 3) IPR013694 - VIT domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAATTAGTGGTGTCTCAGGCGAAGGGCG |
| Internal bar code: | ACTGACTCTAACATTGAGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 432 |
| LEAP-Seq percent confirming: | 96.2121 |
| LEAP-Seq n confirming: | 254 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCTTCTGCTTCTCTTCCT |
| Suggested primer 2: | ACCATGTACTGCTCCTTCCG |