| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.223304 |
| Chromosome: | chromosome 16 |
| Location: | 3460763 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672041 | (1 of 1) 2.1.1.228 - tRNA (guanine(37)-N(1))-methyltransferase / tRNA-(N(1)G37) methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGACCCACTTCCGAGATATGCCCGAGTGC |
| Internal bar code: | GCGCCCCGAAAGGAGGTCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 99.3763 |
| LEAP-Seq n confirming: | 478 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCATGACCAGCGTATGTGA |
| Suggested primer 2: | TGTCGCCTTTTCCATTTACC |