| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.223330 |
| Chromosome: | chromosome 12 |
| Location: | 3601022 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513900 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAACAACGTGTGTGCATCTCTTTATATG |
| Internal bar code: | ATTGGCTCTCCGCTTGCCCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 342 |
| LEAP-Seq percent confirming: | 98.081 |
| LEAP-Seq n confirming: | 920 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCTCCCAGCTGTTCACT |
| Suggested primer 2: | TGCTCTCAGACAGGATGGTG |