Insertion junction: LMJ.RY0402.223348_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.223348
Chromosome:chromosome 17
Location:2379880
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g715050 RAB28 Small Rab-related GTPase; (1 of 1) IPR001806//IPR003579//IPR020849//IPR027417 - Small GTPase superfamily // Small GTPase superfamily, Rab type // Small GTPase superfamily, Ras type // P-loop containing nucleoside triphosphate hydrolase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCCATAGCAGTAGAGGCCCACTCCATGGC
Internal bar code:CCTACTCTTACAAATGGGGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:342
LEAP-Seq percent confirming:99.3506
LEAP-Seq n confirming:306
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTTGCCTCGCTTTCATTT
Suggested primer 2:AGCTGGCAGGCGTAGAAGT