Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.223361 |
Chromosome: | chromosome_8 |
Location: | 3904027 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g378200 | PPO1 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACACACACACAACACACAAACACGCACAGG |
Internal bar code: | CGCAAGTTATCGCGTCTTTTAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 552 |
LEAP-Seq percent confirming: | 67.0848 |
LEAP-Seq n confirming: | 3316 |
LEAP-Seq n nonconfirming: | 1627 |
LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGTCCAAACAACAGCCACC |
Suggested primer 2: | GCACCTGTACCACTTCGGAT |