Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.223361 |
Chromosome: | chromosome_8 |
Location: | 3904029 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g378200 | PPO1 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AAAAAAGACGAAGCCCGCCCATGTGTGTGT |
Internal bar code: | ACCAGGGGATCAGGCGGACGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 331 |
LEAP-Seq percent confirming: | 46.3602 |
LEAP-Seq n confirming: | 13291 |
LEAP-Seq n nonconfirming: | 15378 |
LEAP-Seq n unique pos: | 109 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGTCCAAACAACAGCCACC |
Suggested primer 2: | GCACCTGTACCACTTCGGAT |