| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.223402 |
| Chromosome: | chromosome 14 |
| Location: | 3622240 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631250 | (1 of 1) PTHR12832:SF11 - PROTEIN M05D6.2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACAATCAGGCGCTTCTCCCGCCCTGCC |
| Internal bar code: | TGCTTGGCGGCCCTATAGATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 30 |
| LEAP-Seq percent confirming: | 98.7805 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGGATCGTTTTGTTCTT |
| Suggested primer 2: | ACGGTAGTGAACCCAGAACG |