| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.223410 |
| Chromosome: | chromosome 1 |
| Location: | 3950925 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025750 | (1 of 1) PF01551 - Peptidase family M23 (Peptidase_M23) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTTGGAGCTCCAATGAGCAGGCAACCTA |
| Internal bar code: | ATCCGGAGGATCGACGAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 590 |
| LEAP-Seq percent confirming: | 98.8189 |
| LEAP-Seq n confirming: | 251 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTAGCAACCGGTACCAAC |
| Suggested primer 2: | AGCCCAACTGGTACGACATC |