Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.223410 |
Chromosome: | chromosome_1 |
Location: | 3950925 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g025750 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCTTGGAGCTCCAATGAGCAGGCAACCTA |
Internal bar code: | ATCCGGAGGATCGACGAGGGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 590 |
LEAP-Seq percent confirming: | 98.8189 |
LEAP-Seq n confirming: | 251 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCCTAGCAACCGGTACCAAC |
Suggested primer 2: | AGCCCAACTGGTACGACATC |