Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.223428 |
Chromosome: | chromosome_16 |
Location: | 2818473 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g663200 | FAP295 | Flagellar Associated Protein, cyclic nucleotide dependent protei | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCTCAATTAGGCATTTGGCCGCTGCACGA |
Internal bar code: | GCGAAGTTTGGTACAGCACTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 309 |
LEAP-Seq percent confirming: | 97.1945 |
LEAP-Seq n confirming: | 4088 |
LEAP-Seq n nonconfirming: | 118 |
LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATTTCTCGGTTGTTGGAACG |
Suggested primer 2: | AGGACTGACGATGTGAACCC |