| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.223429 |
| Chromosome: | chromosome 2 |
| Location: | 5440148 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107600 | CGL118,GAK1 | UDP-D-glucuronate kinase; (1 of 1) 2.7.1.43 - Glucuronokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGTGCATGGCTGCCTTGGGCACCTGAGT |
| Internal bar code: | GGTTGAGTCGGCGAGGACGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 325 |
| LEAP-Seq percent confirming: | 98.5088 |
| LEAP-Seq n confirming: | 1123 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGAATGACTCTTGCACCA |
| Suggested primer 2: | CCCACCGGAGTATATGATGG |