| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.223476 |
| Chromosome: | chromosome 1 |
| Location: | 771420 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004000 | (1 of 1) PF08855 - Domain of unknown function (DUF1825) (DUF1825) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCGCTCCTCTTCACACGAGGAGATGTA |
| Internal bar code: | GTACTTGGCCGTTGCTGTTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 482 |
| LEAP-Seq percent confirming: | 99.4639 |
| LEAP-Seq n confirming: | 5380 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGATCTCATAGCGGTCCGA |
| Suggested primer 2: | GTGGGTTAGGCACGGTTCTA |