| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.223478 |
| Chromosome: | chromosome 3 |
| Location: | 1127234 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149050 | (1 of 1) PF03188//PF13415 - Eukaryotic cytochrome b561 (Cytochrom_B561) // Galactose oxidase, central domain (Kelch_3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGTGCAGATGCGGGTATAGGAAGGTGG |
| Internal bar code: | TCCGTACCGTCTTTATTTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1376 |
| LEAP-Seq percent confirming: | 99.6206 |
| LEAP-Seq n confirming: | 6827 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACGTGTGGACCATCCTT |
| Suggested primer 2: | GGAACGTGCAGTCCTCTCTC |