| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.223482 |
| Chromosome: | chromosome 16 |
| Location: | 1569111 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653700 | (1 of 1) PTHR10972//PTHR10972:SF71 - OXYSTEROL-BINDING PROTEIN-RELATED // OXYSTEROL-BINDING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCCGGCAGCTCGCCCCAGGCCTTCTTCT |
| Internal bar code: | GGTAAGCCCTCGGTTTTGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 834 |
| LEAP-Seq percent confirming: | 96.3318 |
| LEAP-Seq n confirming: | 1707 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGGCTTCATTATGTTGTT |
| Suggested primer 2: | TTGTGTGCAGAGTCTGGAGG |