| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.223485 |
| Chromosome: | chromosome_13 |
| Location: | 3367032 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g586750 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AATACTAGACCCGACCGTGCTTCAAGGCCT |
| Internal bar code: | GTTTGGGCGGCGACTTGGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 605 |
| LEAP-Seq percent confirming: | 99.3756 |
| LEAP-Seq n confirming: | 1114 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACCACTGGCTGCAAAACT |
| Suggested primer 2: | CAATACAATGCAAACGCACC |