| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.223507 |
| Chromosome: | chromosome 9 |
| Location: | 6212378 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g406050 | DIV64,PYR7 | (1 of 1) 6.3.4.2 - CTP synthase (glutamine hydrolyzing) / UTP--ammonia ligase; CTP synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGATCTGCCTCCTCTACCTCCATCCCG |
| Internal bar code: | CCATAAGATTTTAATCGGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 368 |
| LEAP-Seq percent confirming: | 98.5456 |
| LEAP-Seq n confirming: | 17956 |
| LEAP-Seq n nonconfirming: | 265 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTGCTGCTGCTGTGGAG |
| Suggested primer 2: | GCGCCGAATAACGTTGTAAT |