| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.223509 |
| Chromosome: | chromosome 8 |
| Location: | 2284078 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370150 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGGCAGTGAGCCCACAGTGCCAACCAC |
| Internal bar code: | GGAGATCCATACAGAGGAAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 81.3472 |
| LEAP-Seq n confirming: | 628 |
| LEAP-Seq n nonconfirming: | 144 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATCTAGCGATCCCCTCCC |
| Suggested primer 2: | CCTGGCTGCTGCTATCTACC |