| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.223596 |
| Chromosome: | chromosome 9 |
| Location: | 7859001 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g416850 | POB32 | Proteome of basal body 32; (1 of 3) IPR000104//IPR001763 - Antifreeze protein, type I // Rhodanese-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGGCTCCAACAGCCGCTCCAGCTCTCC |
| Internal bar code: | GCCTGGAGTGACCGGGTCCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 111 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGTGTGTTTTCCCTTGT |
| Suggested primer 2: | GTCGCCACCAAAGAACTTGT |