| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.223655 |
| Chromosome: | chromosome 13 |
| Location: | 3430794 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587376 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCATGATCCTGCAAACAGTCCAGCAGCG |
| Internal bar code: | ATTGGGGTTAATTTGGCATGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1183 |
| LEAP-Seq percent confirming: | 99.2685 |
| LEAP-Seq n confirming: | 8956 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATTCGCTCAACTAGGGCT |
| Suggested primer 2: | GTTGCAAGCAGCAGCAATTA |